SCIENCE AT A GLANCE

Integrated analysis of natural glycans using a versatile pyrazolone-type heterobifunctional tag ANPMP

Glycans mediate various biological processes through carbohydrate-protein interactions, and glycan microarrays have become indispensable tools for understanding these mechanisms. However, advances in functional glycomics are hindered by the absence of convenient and universal methods for obtaining natural glycan libraries with diverse structures from glycoconjugates. A Chinese research group develops an integrative approach that enables one pot release and simultaneously capture, separation, structural characterization, and functional analysis of N/O-glycans basing on a novel approach for preparing glycan-AEPMP conjugates. A shotgun natural glycan microarray is developed which enables the exploration of carbohydrate-protein interactions and potential biomarkers in cancer research.

Original publication : https://doi.org/10.1016/j.carbpol.2023.121617

#ANPMPtag #Naturalglycan #Glycanmicroarray #Microarray scanner