SCIENCE AT A GLANCE Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins In contrast to exosomes, glycomic studies of microvesicles are largely absent. This study presents a comprehensive analysis of the glycoprofiles of microvesicles (MVs) derived from endothelial EA.hy 926 cells, by plant lectins. The glycan profiles of the MVs…
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SCIENCE AT A GLANCE Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics Immunoglobulin G One of the most common antibodies presented primarily in the blood and tissues, plays a significant role in physiological and pathological activities. It has been discovered that IgG comprises a conserved N-glycosylation site at Asn297 in…
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SCIENCE AT A GLANCE Epistasis mediates the evolution of the receptor binding mode in recent human H3N2 hemagglutinin Since the 1968 “Hong Kong flu” pandemic, H3N2 subtype of influenza A virus has been circulating in human for over half a century. As the major antigen of influenza virus, hemagglutinin (HA) glycoprotein is the fastest evolving…
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SCIENCE AT A GLANCE Decoding complexity in biomolecular recognition of DNA i-motifs with microarrays The three-dimensional structure of the genome has a major impact on gene expression. Predominantly, the human genome consists of duplex B-form DNA though it can also adopt several non-B conformations including DNA intercalated-motif (i-Motif). i-Motifs are formed by C-rich DNA sequences…
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SCIENCE AT A GLANCE Anti-neuronal antibodies against brainstem antigens are associated with COVID-19 Understanding how SARS-CoV-2 affects respiratory centers in the brainstem may help to preclude assisted ventilation for patients in intensive care setting. Researchers used peptide microarrays to screen serum and CSF samples from COVID-19 patients admitted to intensive care and compared with controls…
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SCIENCE AT A GLANCE FAK suppresses antigen processing and presentation to promote immune evasion in pancreatic cancer Pancreatic ductal adenocarcinoma (PDAC) is generally regarded as an immunologically ‘cold’ tumor type, devoid of CD8 T- cell infiltration and unresponsive to single- agent immunotherapy. Findings identify a novel role for focal adhesion kinase （FAK） in regulating the antigenicity…
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SCIENCE AT A GLANCE Cyanostrainchip : a Novel DNA Microarray Tool for High-Throughput Detection of Environmental Cyanobacteria at the Strain Level Cyanobacteria in environments is an important concern due to their crucial roles in ecosystems. A novel DNA microarray tool, termed CyanoStrainChip, is developed which enables strain-level comprehensive profiling of environmental cyanobacteria. Nearly all major…
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SCIENCE AT A GLANCE Evolution of human H3N2 influenza virus Receptor specificity has substantially expanded the receptor-binding domain site Hemagglutinins (HAs) from human influenza viruses adapt to glycans on human airway cells to transmit within the human population. Glycan microarrays comprising various N- and O-linked glycans and glycolipids provide insights into the receptor-binding specificities of…
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SCIENCE AT A GLANCE Integrated analysis of natural glycans using a versatile pyrazolone-type heterobifunctional tag ANPMP Glycans mediate various biological processes through carbohydrate-protein interactions, and glycan microarrays have become indispensable tools for understanding these mechanisms. However, advances in functional glycomics are hindered by the absence of convenient and universal methods for obtaining natural glycan libraries…
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SCIENCE AT A GLANCE Machine-learning Assisted Influenza Vaccine Strain Selection framework Vaccines are the main pharmaceutical intervention used against the global public health threat posed by influenza viruses. Machine-learning Assisted Influenza VaccinE Strain Selection framework, MAIVeSS, can potentially reduce the optimal vaccine candidate selection time from months to days and thus facilitate timely supply of…
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