SCIENCE AT A GLANCE

Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

In contrast to exosomes, glycomic studies of microvesicles are largely absent. This study presents a comprehensive analysis of the glycoprofiles of microvesicles (MVs) derived from endothelial EA.hy 926 cells, by plant lectins. The glycan profiles of the MVs were analyzed using a solid-phase assay. In this assay, MVs were immobilized on glass slides, allowing for the examination of their glycan interactions with 21 plant lectins. The binding of lectins to the immobilized MVs was then assessed with InnoScan 1100 AL microarray scanner. Results indicate the glycosylation patterns of MVs differ markedly from those of their parent cells, with a predominance of oligolactosamines and their α2-6-sialylated forms as N-glycans, while levels of α2-3-sialylated glycans were notably low. This study provides valuable insights into the glycan composition of endothelial cell-derived MVs and their potential biological significance.

Original publication : https://doi.org/10.3390/ijms25115725

#Microvesicle #Glycan #Microarray